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glutamate decarboxylase gad67  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology glutamate decarboxylase gad67
    Fig. 5. Effects of exercise training on the 67-kDa isoform of glutamate decarboxylase <t>(GAD67)</t> immunoreactivity and neuronal activity in the RVLM of NS rats and HS rats. (A and C) A representative immunohistochemistry image and the column diagram showing the effects of exercise training on the positive neurons of GAD67 (brown yellow) in the RVLM of NS rats and HS rats. (B and D) A representative immunohistochemistry image and the column diagram showing the effects of exercise training on the Fra-LI (brown dots, an indicator of chronic neuronal excitation) expression in the RVLM of NS rats and HS rats. Values are mean ± SE. *P<0.05 vs. NS groups; †P<0.05 HS + Ex vs. HS + Sed.
    Glutamate Decarboxylase Gad67, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gad67+activity/GAD-67+Lentiviral+Activation+Particles/pm30048986-94-51-54
    Average 88 stars, based on 1 article reviews
    glutamate decarboxylase gad67 - by Bioz Stars, 2026-09
    88/100 stars

    Images

    1) Product Images from "Exercise Training Attenuates Proinflammatory Cytokines, Oxidative Stress and Modulates Neurotransmitters in the Rostral Ventrolateral Medulla of Salt-Induced Hypertensive Rats."

    Article Title: Exercise Training Attenuates Proinflammatory Cytokines, Oxidative Stress and Modulates Neurotransmitters in the Rostral Ventrolateral Medulla of Salt-Induced Hypertensive Rats.

    Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

    doi: 10.1159/000492095

    Fig. 5. Effects of exercise training on the 67-kDa isoform of glutamate decarboxylase (GAD67) immunoreactivity and neuronal activity in the RVLM of NS rats and HS rats. (A and C) A representative immunohistochemistry image and the column diagram showing the effects of exercise training on the positive neurons of GAD67 (brown yellow) in the RVLM of NS rats and HS rats. (B and D) A representative immunohistochemistry image and the column diagram showing the effects of exercise training on the Fra-LI (brown dots, an indicator of chronic neuronal excitation) expression in the RVLM of NS rats and HS rats. Values are mean ± SE. *P<0.05 vs. NS groups; †P<0.05 HS + Ex vs. HS + Sed.
    Figure Legend Snippet: Fig. 5. Effects of exercise training on the 67-kDa isoform of glutamate decarboxylase (GAD67) immunoreactivity and neuronal activity in the RVLM of NS rats and HS rats. (A and C) A representative immunohistochemistry image and the column diagram showing the effects of exercise training on the positive neurons of GAD67 (brown yellow) in the RVLM of NS rats and HS rats. (B and D) A representative immunohistochemistry image and the column diagram showing the effects of exercise training on the Fra-LI (brown dots, an indicator of chronic neuronal excitation) expression in the RVLM of NS rats and HS rats. Values are mean ± SE. *P<0.05 vs. NS groups; †P<0.05 HS + Ex vs. HS + Sed.

    Techniques Used: Activity Assay, Immunohistochemistry, Expressing

    Fig. 7. Protein expression of TNF-α, GAD67, gp91phox and NOX4 in the RVLM and ANP and β-MHC in the heart of NS rats and HS rats. (A) HS rats had higher levels of TNF-α and lower levels of GAD67 in the RVLM when compared with NS rats. Exercise training decreased the protein expression of TNF-α and increased the protein express of GAD67 in the RVLM of HS rats. (B) HS rats had higher levels of gp91phox and NOX4 in the RVLM when compared with NS rats. Exercise training decreased the protein expression of gp91phox and NOX4 in the RVLM when compared with NS rats. (C) Western blot for ANP and β-MHC expressions in the heart were lower in exercise training-treated HS rats than in sedentary HS rats. Values are mean ± SE. *P<0.05 vs. NS groups; †P<0.05 HS + Ex vs. HS + Sed.
    Figure Legend Snippet: Fig. 7. Protein expression of TNF-α, GAD67, gp91phox and NOX4 in the RVLM and ANP and β-MHC in the heart of NS rats and HS rats. (A) HS rats had higher levels of TNF-α and lower levels of GAD67 in the RVLM when compared with NS rats. Exercise training decreased the protein expression of TNF-α and increased the protein express of GAD67 in the RVLM of HS rats. (B) HS rats had higher levels of gp91phox and NOX4 in the RVLM when compared with NS rats. Exercise training decreased the protein expression of gp91phox and NOX4 in the RVLM when compared with NS rats. (C) Western blot for ANP and β-MHC expressions in the heart were lower in exercise training-treated HS rats than in sedentary HS rats. Values are mean ± SE. *P<0.05 vs. NS groups; †P<0.05 HS + Ex vs. HS + Sed.

    Techniques Used: Expressing, Western Blot

    Related Articles

    De-Phosphorylation Assay:

    Article Title: POSTTRANSLATIONAL MODIFICATION OF GLUTAMIC ACID DECARBOXYLASE 67 BY INTERMITTENT HYPOXIA: Evidence for the involvement of dopamine D1 receptor signaling
    Article Snippet: .. Following dephosphorylation, the reaction mixtures were assayed for GAD67 activity as described above. siRNA transfection Transfection of PC12 cells with siRNAs specific for either D1R or scrambled sequence (control) was performed at a concentration of 60 picomoles per millilitre by following the procedure recommended by the manufacturer (Santa Cruz Biotechnology, Santa Cruz, CA). ..

    Activity Assay:

    Article Title: POSTTRANSLATIONAL MODIFICATION OF GLUTAMIC ACID DECARBOXYLASE 67 BY INTERMITTENT HYPOXIA: Evidence for the involvement of dopamine D1 receptor signaling
    Article Snippet: .. Following dephosphorylation, the reaction mixtures were assayed for GAD67 activity as described above. siRNA transfection Transfection of PC12 cells with siRNAs specific for either D1R or scrambled sequence (control) was performed at a concentration of 60 picomoles per millilitre by following the procedure recommended by the manufacturer (Santa Cruz Biotechnology, Santa Cruz, CA). ..

    Transfection:

    Article Title: POSTTRANSLATIONAL MODIFICATION OF GLUTAMIC ACID DECARBOXYLASE 67 BY INTERMITTENT HYPOXIA: Evidence for the involvement of dopamine D1 receptor signaling
    Article Snippet: .. Following dephosphorylation, the reaction mixtures were assayed for GAD67 activity as described above. siRNA transfection Transfection of PC12 cells with siRNAs specific for either D1R or scrambled sequence (control) was performed at a concentration of 60 picomoles per millilitre by following the procedure recommended by the manufacturer (Santa Cruz Biotechnology, Santa Cruz, CA). ..

    Sequencing:

    Article Title: POSTTRANSLATIONAL MODIFICATION OF GLUTAMIC ACID DECARBOXYLASE 67 BY INTERMITTENT HYPOXIA: Evidence for the involvement of dopamine D1 receptor signaling
    Article Snippet: .. Following dephosphorylation, the reaction mixtures were assayed for GAD67 activity as described above. siRNA transfection Transfection of PC12 cells with siRNAs specific for either D1R or scrambled sequence (control) was performed at a concentration of 60 picomoles per millilitre by following the procedure recommended by the manufacturer (Santa Cruz Biotechnology, Santa Cruz, CA). ..

    Control:

    Article Title: POSTTRANSLATIONAL MODIFICATION OF GLUTAMIC ACID DECARBOXYLASE 67 BY INTERMITTENT HYPOXIA: Evidence for the involvement of dopamine D1 receptor signaling
    Article Snippet: .. Following dephosphorylation, the reaction mixtures were assayed for GAD67 activity as described above. siRNA transfection Transfection of PC12 cells with siRNAs specific for either D1R or scrambled sequence (control) was performed at a concentration of 60 picomoles per millilitre by following the procedure recommended by the manufacturer (Santa Cruz Biotechnology, Santa Cruz, CA). ..

    Concentration Assay:

    Article Title: POSTTRANSLATIONAL MODIFICATION OF GLUTAMIC ACID DECARBOXYLASE 67 BY INTERMITTENT HYPOXIA: Evidence for the involvement of dopamine D1 receptor signaling
    Article Snippet: .. Following dephosphorylation, the reaction mixtures were assayed for GAD67 activity as described above. siRNA transfection Transfection of PC12 cells with siRNAs specific for either D1R or scrambled sequence (control) was performed at a concentration of 60 picomoles per millilitre by following the procedure recommended by the manufacturer (Santa Cruz Biotechnology, Santa Cruz, CA). ..



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    Santa Cruz Biotechnology glutamate decarboxylase gad67
    Fig. 5. Effects of exercise training on the 67-kDa isoform of glutamate decarboxylase <t>(GAD67)</t> immunoreactivity and neuronal activity in the RVLM of NS rats and HS rats. (A and C) A representative immunohistochemistry image and the column diagram showing the effects of exercise training on the positive neurons of GAD67 (brown yellow) in the RVLM of NS rats and HS rats. (B and D) A representative immunohistochemistry image and the column diagram showing the effects of exercise training on the Fra-LI (brown dots, an indicator of chronic neuronal excitation) expression in the RVLM of NS rats and HS rats. Values are mean ± SE. *P<0.05 vs. NS groups; †P<0.05 HS + Ex vs. HS + Sed.
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    gad67 activity  (Santa Cruz Biotechnology)
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    Image Search Results


    Fig. 5. Effects of exercise training on the 67-kDa isoform of glutamate decarboxylase (GAD67) immunoreactivity and neuronal activity in the RVLM of NS rats and HS rats. (A and C) A representative immunohistochemistry image and the column diagram showing the effects of exercise training on the positive neurons of GAD67 (brown yellow) in the RVLM of NS rats and HS rats. (B and D) A representative immunohistochemistry image and the column diagram showing the effects of exercise training on the Fra-LI (brown dots, an indicator of chronic neuronal excitation) expression in the RVLM of NS rats and HS rats. Values are mean ± SE. *P<0.05 vs. NS groups; †P<0.05 HS + Ex vs. HS + Sed.

    Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

    Article Title: Exercise Training Attenuates Proinflammatory Cytokines, Oxidative Stress and Modulates Neurotransmitters in the Rostral Ventrolateral Medulla of Salt-Induced Hypertensive Rats.

    doi: 10.1159/000492095

    Figure Lengend Snippet: Fig. 5. Effects of exercise training on the 67-kDa isoform of glutamate decarboxylase (GAD67) immunoreactivity and neuronal activity in the RVLM of NS rats and HS rats. (A and C) A representative immunohistochemistry image and the column diagram showing the effects of exercise training on the positive neurons of GAD67 (brown yellow) in the RVLM of NS rats and HS rats. (B and D) A representative immunohistochemistry image and the column diagram showing the effects of exercise training on the Fra-LI (brown dots, an indicator of chronic neuronal excitation) expression in the RVLM of NS rats and HS rats. Values are mean ± SE. *P<0.05 vs. NS groups; †P<0.05 HS + Ex vs. HS + Sed.

    Article Snippet: Published by S. Karger AG, Basel www.karger.com/cpb Li et al.: Exercise Training in Salt-Induced Hypertension Immunohistochemistry and immunofluorescence studies Immunohistochemical labeling was performed in floating sections (18 μm) as previously described [21, 22] to identify Fra-like (Fra-LI, a marker of chronic neuronal activation), tumour necrosis factor-alpha (TNF-α) and 67-kDa isoform of glutamate decarboxylase (GAD67) (Santa Cruz Biotechnology, Santa Cruz, California) expressions.

    Techniques: Activity Assay, Immunohistochemistry, Expressing

    Fig. 7. Protein expression of TNF-α, GAD67, gp91phox and NOX4 in the RVLM and ANP and β-MHC in the heart of NS rats and HS rats. (A) HS rats had higher levels of TNF-α and lower levels of GAD67 in the RVLM when compared with NS rats. Exercise training decreased the protein expression of TNF-α and increased the protein express of GAD67 in the RVLM of HS rats. (B) HS rats had higher levels of gp91phox and NOX4 in the RVLM when compared with NS rats. Exercise training decreased the protein expression of gp91phox and NOX4 in the RVLM when compared with NS rats. (C) Western blot for ANP and β-MHC expressions in the heart were lower in exercise training-treated HS rats than in sedentary HS rats. Values are mean ± SE. *P<0.05 vs. NS groups; †P<0.05 HS + Ex vs. HS + Sed.

    Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

    Article Title: Exercise Training Attenuates Proinflammatory Cytokines, Oxidative Stress and Modulates Neurotransmitters in the Rostral Ventrolateral Medulla of Salt-Induced Hypertensive Rats.

    doi: 10.1159/000492095

    Figure Lengend Snippet: Fig. 7. Protein expression of TNF-α, GAD67, gp91phox and NOX4 in the RVLM and ANP and β-MHC in the heart of NS rats and HS rats. (A) HS rats had higher levels of TNF-α and lower levels of GAD67 in the RVLM when compared with NS rats. Exercise training decreased the protein expression of TNF-α and increased the protein express of GAD67 in the RVLM of HS rats. (B) HS rats had higher levels of gp91phox and NOX4 in the RVLM when compared with NS rats. Exercise training decreased the protein expression of gp91phox and NOX4 in the RVLM when compared with NS rats. (C) Western blot for ANP and β-MHC expressions in the heart were lower in exercise training-treated HS rats than in sedentary HS rats. Values are mean ± SE. *P<0.05 vs. NS groups; †P<0.05 HS + Ex vs. HS + Sed.

    Article Snippet: Published by S. Karger AG, Basel www.karger.com/cpb Li et al.: Exercise Training in Salt-Induced Hypertension Immunohistochemistry and immunofluorescence studies Immunohistochemical labeling was performed in floating sections (18 μm) as previously described [21, 22] to identify Fra-like (Fra-LI, a marker of chronic neuronal activation), tumour necrosis factor-alpha (TNF-α) and 67-kDa isoform of glutamate decarboxylase (GAD67) (Santa Cruz Biotechnology, Santa Cruz, California) expressions.

    Techniques: Expressing, Western Blot

    Table 2

    Journal:

    Article Title: POSTTRANSLATIONAL MODIFICATION OF GLUTAMIC ACID DECARBOXYLASE 67 BY INTERMITTENT HYPOXIA: Evidence for the involvement of dopamine D1 receptor signaling $

    doi: 10.1111/j.1471-4159.2010.07063.x

    Figure Lengend Snippet: Table 2

    Article Snippet: Following dephosphorylation, the reaction mixtures were assayed for GAD67 activity as described above. siRNA transfection Transfection of PC12 cells with siRNAs specific for either D1R or scrambled sequence (control) was performed at a concentration of 60 picomoles per millilitre by following the procedure recommended by the manufacturer (Santa Cruz Biotechnology, Santa Cruz, CA).

    Techniques: De-Phosphorylation Assay, Activity Assay